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Using a similar experimental design as in the DIO dCas9 VPR experiments Figure 1, we transfected HEK293T cells with plasmids expressing constitutive dCas9 VPR, SVI dCas9 VPR, and SVI DIO dCas9 VPR constructs, along with sgRNA targeting the GRM2 gene promoter or lacZ control 500 mg augmentin This causes an effect akin to actions of a loop diuretic, with a higher rate of excretion of Na and Cl ions as well as a lower renal medullary interstitial effective osmolality see Chapter 9